Kapa Biosystems is constantly evolving better science by applying the latest advances in genomics and synthetic biology to help reduce costs, simplify workflows and improve results. Kapa’s core protein engineering technologies enable them to tailor enzymes to suit specific applications in next-generation DNA and RNA sequencing, DNA amplification and molecular diagnostics. Biomedical research is no longer limited by the constraints of native enzyme structure or function.

Evolved for extreme fidelity, robustness, and low-bias amplification of difficult templates.

KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. The intrinsic high processivity of KAPA HiFi results in significant improvements to yield, sensitivity, speed, target length, and the ability to amplify difficult templates (e.g AT- and GC-rich). The error rate of KAPA HiFi PCR Kits is 100X lower than wild-type Taq DNA polymerase.

Product Highlights:

• Achieve the highest fidelity
• Improve performance on GC- and AT-rich templates
• Amplify longer targets with greater sensitivity

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Accurate detection of DNA sequence variations.

High Resolution Melt (HRM) analysis is a technique for fast, high-throughput post-PCR analysis of genetic mutations or variance in nucleic acid sequences. The KAPA HRM FAST Master Mix contains a novel DNA polymerase and buffer system, and EvaGreen® saturating dye optimized for maximum discrimination between sequence variants.

Product Highlights:

• Maximize differences in sequence variants
• Accurately detect Type IV SNPs
• Go from cell to SNP quickly

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Evolved to shine.

KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis.

Product Highlights:

• Quantitate changes in gene expression more accurately
• Detect low copy and difficult targets consistently
• Complete real-time PCR runs in just 40 minutes

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Optimized for Versatility and Speed.

KAPA PROBE FAST qPCR Kits provide fast and reproducible results for all probe-based qPCR applications. Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g. TaqMan®), FRET probes, and displacement probes (e.g. molecular beacons).

Product Highlights:

• Accurately discriminate between alleles
• Achieve excellent reproducibility and efficiency
• Increase flexibility

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Evolved to solve.

The second-generation KAPA2G Robust DNA Polymerase was evolved to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). This product is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.

Product Highlights:

• Efficient amplification of GC- and AT-rich targets
• Improved tolerance to common PCR inhibitors
• Unrivalled performance in colony PCR

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One Evolved Polymerase. One Step. One Tube.

KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kit is optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination with a convenient qRT-PCR protocol.

Product Highlights:

• Increase signal and reaction efficiency
• Amplify across a broad range of target lengths
• Improve sensitivity and reproducibility

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For Next-Generation Sequencing.

KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification. Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR.

Product Highlights:

• Accurately quantify PCR-competent sequencing templates
• Improve pooling for multiplexed sequencing
• Eliminate drift with carefully quality-controlled DNA standards

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The only evolved DNA Polymerase for Plant PCR

KAPA3G Plant PCR Kits are designed for PCR of plant-derived DNA, using either purified DNA or DNA prepared by crude extraction methods (crude sample PCR). In addition, the KAPA3G Plant PCR Kit can be used to amplify DNA from plant material added directly to the PCR (direct PCR).

Product Highlights:

• Amplify long targets from crude samples nd purified DNA
• Perform direct PCR from a variety of plant species and tissue types
• Streamline workflows and improve turnaround times
• Improve success rates with novel crude samples plant PCR workflow

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